| | Клуб | Менеджер | Ліга | Стадіон | Місцезнаходження | Заснований |
 | Carolina Core FC | | MLS Next Pro | Truist Point | High Point | 2022 |
| FC Baltimore Christos | | National Premier Soccer League | Marriotts Ridge High School | Marriottsville, Maryland | 2018 |
| Atlanta United 2 | Steve Cooke | USL Championship | Coolray Field | Lawrenceville | 2017 |
| FC Tucson | | USL League One | Kino Sports Complex | Tucson | 2010 |
| Milwaukee Torrent | | National Premier Soccer League | Hart Park | Milwaukee | 2015 |
| Portland Timbers 2 | Cameron Knowles | | Merlo Field | Portland | 2015 |
| Rochester Rhinos | | | Sahlen's Stadium | Rochester | 1996 |
| Peachtree City MOBA | | | MOBA Soccer Stadium | Peachtree, Georgia | 2013 |
| Virginia Cavalry FC | | | Edelman Financial Field | Ashburn, Virginia | 2012 |
| California United | | | Titan Stadium | Fullerton | 2017 |
| Real Monarchs SLC | Jámison Olave | USL Championship | Rio Tinto Stadium | Sandy | 2014 |
| New York RB 2 | Ibrahim Sekagya | USL Championship | Columbia Soccer Stadium | New York | 2015 |
| Atlanta SC | Steve Guppy | | Atlanta Silverbacks Park | Atlanta | 1995 |
| Tacoma Defiance | | USL Championship | Cheney Stadium | Seattle | 2014 |
| Jacksonville Armada | | National Premier Soccer League | Baseball Grounds of Jacksonville | Jacksonville | 2013 |
| Chivas USA | | | Dignity Health Sports Park | Los Angeles | 2004 |
| Inter Miami CF II | Cristian Ledesma | USL League One | DRV PNK Stadium | Miami | 2019 |
| Wilmington Hammerheads | | | Legion Stadium | Wilmington | 1996 |
| San Antonio Scorpions | | | Heroes Stadium | San Antonio | 2011 |
| Penn FC | | | FNB Field | Harrisburg | 2003 |
| Charlotte Eagles | | | Charlotte Christian School | Charlotte | 1991 |
| Dayton Dutch Lions | Matt Weston | | Miami Valley South Stadium | Dayton | 2009 |
| Phoenix FC | | | Sun Devil Soccer Stadium | Phoenix | 2012 |
| VSI Tampa Bay | | | Plant City Stadium | Plant City | 2011 |
| Ventura County FC | | USL Championship | Dignity Health Sports Park | Los Angeles | 2014 |
| Austin Aztex | | | House Park | Austin | 2008 |
| Saint Louis FC | Steve Trittschuh | | St. Louis Soccer Park | St. Louis | 2014 |
| Rayo OKC | | | Miller Stadium | Oklahoma City | 2015 |
| Sporting KC II | | USL Championship | Children's Mercy Park | Kansas City | 2015 |
| Orlando City B | Marcelo Neveleff | | Titan Soccer Complex | Melbourne | 2015 |
| Philadelphia Union-2 | | | Goodman Stadium | Bethlehem | 2015 |
| San Francisco Deltas | | | Kezar Stadium | San Francisco | 2016 |
| Reno 1868 FC | Ian Russell | | Greater Nevada Field | Reno | 2015 |
| Long Island Rough Riders | | | Hofstra University Soccer Stadium | South Huntington | 1994 |
| Lansing Ignite | | | Cooley Law School Stadium | Lansing | 2018 |
| North Texas SC | | USL League One | Toyota Stadium | Frisco | 2018 |
| FC Miami City | | | Central Broward Park | Miami | 2014 |
| New England Revolution II | | USL League One | Gillette Stadium | Boston | 2019 |
| Denton Diablos | | National Premier Soccer League | Mean Green Soccer Stadium | Denton, Texas | 2018 |
| Queensboro FC | | USL Championship | York College | New York City | 2019 |
| Fort Worth Vaqueros FC | | National Premier Soccer League | Farrington Field | Fort Worth, Texas | 2013 |
| Corpus Christi FC | | | Jack Dugan Family Soccer and Track Stadium | Corpus Christi | 2017 |
| Austin FC II | | MLS Next Pro | Parmer Field | Austin | 2022 |
| Crown Legacy FC | | MLS Next Pro | Sportsplex at Matthews | Matthews | 2022 |
| Chicago Fire II | | MLS Next Pro | SeatGeek Stadium | Bridgeview | 2021 |
| Colorado Rapids 2 | | MLS Next Pro | Denver Soccer Stadium | Denver | 2021 |
| Columbus Crew 2 | | MLS Next Pro | Historic Crew Stadium | Columbus | 2021 |
| FC Cincinnati 2 | Tyrone Marshall | MLS Next Pro | NKU Soccer Stadium | Cincinnati | 2021 |
| Houston Dynamo 2 | | MLS Next Pro | SaberCats Stadium | Houston | 2021 |
| Los Angeles FC 2 | | MLS Next Pro | Titan Stadium | Los Angeles | 2022 |
